| (a) Schematic of iMuSCs isolation method from injured murine TA muscles. (b) Bright field images of uninjured and injured cultures. 3 days after the cell isolation no cells appeared in the control uninjured cultures, but iMuSCs were present in the injured cultures. 7 days after cell isolation, the proliferation of iMuSCs was apparent. Scale bar = 10 μm. (c) Msx1 (green), Pax7 (red), Cxcr4 (green), and Sca1 (red) expression of iMuSCs. Nuclei were stained with DAPI (blue). Scale bar = 100 μm. (d) qPCR analysis of whole biopsied TA muscles, and (e), fresh isolated iMuSCs. (f) Single cell migration pathways of iMuSCs, and the control C2C12 and MuSCs. The migration paths of 20 individual cells from different experimental groups captured in a time-lapse motility assay. Data was pooled from 3 independent experiments. Graphs show the calculated accumulated distance and velocity of the cells. Data are represented as the mean ±SEM of 60 individual cells from 3 biological replicates. **P < 0.01. (g) qPCR analysis of β-Catenin, E-Cadherin, M-Cadherin, N-Cadherin expression of iMuSCs. Data are represented as the mean ±SEM of 5 biological replicates. |
